Immortalized Mouse Myeloid-derived Suppressor-like (HD1A) Cells

Peritoneal macrophages were collected from wild type male mice that had been crossbred with Immortomouse expressing a temperature-sensitive version of simian virus 40 large T antigen to generate the Immortalized Mouse Myeloid-derived Suppressor-like (HD1A) Cells. Myeloid-derived suppressor cells are myeloid progenitors that are blocked to further differentiate and may have a role in tumor proliferation. The cells are the wild type to Stable Mouse Myeloid-derived Suppressor-like LAL Knock Out (HD1B) Cell Line (available at abm, T3187), in which lysopmal acid lipase (LAL) expression is observed. Together HD1A and HD1B cells may be used as an in vitro system for studies relating to LAL and myeloid functions.

Immortalization Method: Immortomouse expressing a temperature-sensitive version of simian virus 40 large T antigen

BioSafety Level: II

Species: Mouse

Source Organ: Peritoneal

Growth Properties: Adherent

Morphology: Polygonal

Population Doubling: N/A

Seeding Density: Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers: N/A

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow II medium available in abm, Cat. No. TM002. To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to final concentration of 10%, and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: N/A

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C