Immortalized Mouse Mutant Epidermal Keratinocytes (CDC25A 8b)

Cell division cycle 25 (CDC25) family of phosphatases consists of CDC25A, CDC25B and CDC25C in which regulates the cell cycle progression, and thus are important for the activation of cell cycle checkpoints in response to DNA damage. CDC25A degradation has been associated with cell cycle arrest. Immortalized Mouse Mutant Epidermal Keratinocytes (CDC25A 8b) Cells were isolated from the Cdc25a^fl/fl/Cre⁺ mice in which the CDC25A gene is flanked with loxP inserted at exons 1-3 with the Krt14 promoter-driven Cre recombinase (Cre⁺) in which the expression of CDC25A is absent. abm also provides the Immortalized Mouse Epidermal Keratinocytes (CDC25A F7) Cells (T0299) which may be used as a control cell line. The cells are suitable for studies on the CDC25A gene and its expression in cell cycle progression.

Immortalization Method: Spontaneous

BioSafety Level: II

Species: Mouse

Source Organ: Skin

Growth Properties: Adherent

Morphology: Cobble-stone

Population Doubling: N/A

Seeding Density: Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers: N/A

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow XI medium available at abm, Cat. No. TM011. To make the complete growth medium, add the following components to the base medium: 8% chelexed fetal bovine serum (TM999)*, Penicillin/Streptomycin Solution (G255) to a final concentration of 1% and 5 ng/ml recombinant human FGF7 (Z100595).
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: Immunoblot for the presence of CDC25A was conducted.

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C