Immortalized Mouse Leydig Cells (D-4-4) are derived from MT-PVLT-10 transgenic mice expressing polyomavirus large T antigen. The cells are immortalized and are large with extensive cytoplasmic elongations in appearance. The morphology changes at higher densities exhibiting a cuboidal appearance. The Immortalized Mouse Leydig Cells (D-4-4) are a renewable tool for studies in Leydig biology.
Immortalization Method: Derived from transgenic mice expressing polyomavirus large T antigen
BioSafety Level: II
Species: Mouse
Source Organ: Testicle
Growth Properties: Adherent
Morphology: Polygonal
Population Doubling: N/A
Seeding Density: Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Markers: 3β-HSD
Donor Age: N/A
Donor Gender: N/A
Donor Ethnicity: N/A
Propagation: The base medium for this cell line is Prigrow III medium available at abm (TM003). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to final concentration of 5% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: +37.0°C.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.
Quality Control: 1) 50 µg/ml Gentamycin used for selection. 2) RT-PCR used to detect Leydig cell markers.
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C