Immortalized Mouse Kidney Epithelial Cells

The Immortalized Mouse Kidney Epithelial Cells (HPV E6/E7) is derived by infection of primary cells with lentivirus HPV E6/E7. This cell line maintains its primary phenotype and can be grown extensively. While the Immortalized Mouse Kidney Epithelial Cells have been used to study diabetes, its primary counterpart have been used to study inflammatory diseases, intrinsic kidney repair mechanisms and acute renal failures.

Immortalization Method: Transduction with lentiviral HPV (abm Cat. No. LV617)

BioSafety Level: II

Species: Mouse

Source Organ: Kidney

Growth Properties: Adherent

Morphology: Epithelial

Population Doubling: N/A

Seeding Density: 7,000-10,000 cells/cm²; Recommended split ratio is 1:2 to 1:3

Markers: N/A

Donor Age: 10 day old post natal mice

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow X medium available at abm Cat. No. TM0085. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (https://new.abmgood.com/USDA-Research-Grade-Origin-Fetal-Bovine-Serum-TM999-500.html">TM999) to final concentration of 2.5% and Penicillin/Streptomycin Solution (https://new.abmgood.com/Penicillin/Streptomycin-Solution-G255.html#g255">G255) to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.

Quality Control: Real Time PCR was used to quantify the HPV gene expression in immortalized cell line.

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C