Immortalized Mouse Floxed Fam20c Osteoblast Cells

Immortalized Mouse Floxed Fam20c Osteoblast Cells are a reliable tool to study osteoblasts and the roles in formation, mineralization and repair of the osteoblasts. They carry the floxed Fam20c allele which allows further manipulation on exon 6-9 by Cre recombinase to obtain null Fam20c allele cells. Fam20c expresses the FAM20C kinase which has been associated with bone-related diseases due to mineralization dysfunction. The Immortalized Mouse Floxed Fam20c Osteoblast Cells can be expanded in vitro for research on the field of mineralization disorders.

Immortalization Method: Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen

BioSafety Level: II

Species: Mouse

Source Organ: Calvarial bones

Growth Properties: Adherent

Morphology: Polygonal

Population Doubling: N/A

Seeding Density: 30,000-40,000 cells/cm²; Recommend split ratio is 1:2 to 1:4

Markers: N/A

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow III medium available at abm (TM003). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: Immunohistochemistry for gene expression associated with the differentiation and mineralization of osteoblasts

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C