Immortalization Method: Immortalized with retrovirus containing SV40 large T antigen
BioSafety Level: II
Organism: Mouse (M. musculus)
Species: Mouse
Source Organs: Embryo
Organ Type: Embryo
Growth Properties: Adherent
Morphology: Mesenchymal stem cell-like
Markers: Ng2 (Cspg4), Cd146 (Mcam), PdgfrbNeomycin resistance
Propagation:Use of PriCoatTM T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.
The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.Change media every 2-3 days.Carbon dioxide (CO2): 5%, Temperature: +37.0°C.* Do not use heat-inactivated FBS for cell culture unless specified otherwise.
Quality Control: 1) PCR; 2) FACS
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C
Reference: Supakul, S., Yao, K., Ochi, H., Shimada, T., Hashimoto, K., Sunamura, S., Mabuchi, Y., Tanaka, M., Akazawa, C., Nakamura, T., Okawa, A., Takeda, S., & Sato, S. (2019). Pericytes as a Source of Osteogenic Cells in Bone Fracture Healing. International journal of molecular sciences, 20(5), 1079. https://doi.org/10.3390/ijms20051079