Immortalized Mouse Dental Apical Papilla Progenitor Cells (iSCAP)

Description: Immortalized Mouse Dental Apical Papilla Progenitor Cells are hygromycin-resistant, non-tumorigenic reversibly immortalized cells that have retained mesenchymal progenitor markers. The cells are unique, as the immortalization process may be reversed by infection with Cre-recombinase expressing adenoviruses (abm Cat. No. 000018A), thus reverting immortal cells back into primary cells. When transduced with adenovirus expressing BMP9, these cells are able to differentiate into osteogenic, chondrogenic, and adipogenic lineages. Besides expressing osteoblastic/odontoblastic markers such as OPN, OCN, BSP, MEPE, DMP1, DSPP, and ALP, these cells are also able to form mature and mineralized osteoid matrix upon BMP9-transductionThese cells are recommended for use in studies involving apical papilla stem cell biology, and ondontogenic tissue engineering.

Immortalization Method: Serial passaging and transduction with retrovirus SSR#69 expressing SV40 large T antigen flanked with Cre/loxP sites

BioSafety Level: II

Species: Mouse

Source Organ: Lower Incisor Teeth

Growth Properties: Adherent

Morphology: Polygonal

Population Doubling: N/A

Seeding Density: Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers: CD73, CD44, CD90/Thy-1, CD105/Endoglin, CD166/ALCAM, CD133/Prom1, BMPRII, CD117/c-kit, and CD29/integrin β1

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow III medium available at abm (TM003). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.
* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: 1) Immunofluorescence staining performed to examine mesenchymal stem cell markers; 2) sqPCR analysis for BMP9-induced late osteo/odontoblast, and ondotoblast markers.

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C