Immortalization Method: Immortalized with P53 deletion
BioSafety Level: II
Organism: Mouse (M. musculus)
Species: Mouse
Source Organs: Brain
Organ Type: Brain
Growth Properties: Adherent
Morphology: neuronal cell morphology with long body
Propagation: Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow III (TM003) + 2 mM L-glutamine (G275) +10% FBS + 1% Penicillin/Streptomycin Solution (G255), +37.0°C, 5% CO₂
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C
Reference: Thompson, W. E., Branch, A., Whittaker, J. A., Lyn, D., Zilberstein, M., Mayo, K. E., & Thomas, K. (2001). Characterization of Prohibitin in a Newly Established Rat Ovarian Granulosa Cell Line. Endocrinology, 142(9), 4076-4085. doi:10.1210/endo.142.9.8354Sacks, D., Baxter, B., Campbell, B., Carpenter, J. S., Cognard, C., Dippel, D., Eesa, M., Fischer, U., Hausegger, K., Hirsch, J. A., Shazam Hussain, M., Jansen, O., Jayaraman, M. V., Khalessi, A. A., Kluck, B. W., Lavine, S., Meyers, P. M., Ramee, S., Rüfenacht, D. A., … Vorwerk, D. (2018). Multisociety Consensus Quality Improvement Revised Consensus Statement for Endovascular Therapy of Acute Ischemic Stroke. International journal of stroke : official journal of the International Stroke Society, 13(6), 612–632. https://doi.org/10.1177/1747493018778713