Immortalized Mouse Cementoblasts

The cementum is a mineralized tissue lining the tooth root surface and shares many properties with mineralized tissues such as the bone. It has a role in reparative activities, the regeneration of tissues, and is responsible in maintaining healthy periodontium. The Immortalized Mouse Cementoblasts is immortalized with mouse SV40 large T antigen, and retains phenotypic characteristics of the primary cells such as the expression of parathyroid hormone 1 receptor (PTH-1), noncollagenous proteins (BSP, OC, OPN), and type I collagen. Furthermore, this cell line has been shown to respond to the presence of the parathyroid hormone (PTH) and the parathyroid hormone-related protein (PTHrP), and is able to stimulate mineral nodule formation, which makes it a useful tool to study the mechanisms involved in regulating the molecular biological activity and properties of the cementum or mineralized tissues, biomineralization, and affects of the PTH-1 receptor.

Immortalization Method: Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen

BioSafety Level: II

Species: Mouse

Source Organ: Mandibular First Molars

Growth Properties: Adherent

Morphology: Polygonal

Population Doubling: N/A

Seeding Density: Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers: Bone sialoprotein (BSP), Osteocalcin (OC), Osteopontin (OPN), Type I collagen

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow III medium available at abm (TM003). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to final concentration of 10%, Penicillin/Streptomycin Solution (G255).
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: Expression of PTH-1 was assessed by Northern blot and gene-expression assay. Markers were analyzed by RT-PCR.

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C