Immortalized Mouse CD81−/− Macrophage Cells (ASD1) is derived from the long bones of the CD81 -/- mice. The cells were immortalized with the SV40 large T antigen and underwent limiting dilution cloning. The cell line offers users the ability to conduct experiments with a reliable source of immortal cells in vitro. The CD81 gene has associations with malignancies as it is localized within the tumor-suppressor gene region. Hence valuable for studies in regards to cancer. abm also offers the Immortalized Mouse CD81+/− Macrophage Cells (2BSD1.10), Cat. No. T0139, which may be used as a wild type for experiments.
Immortalization Method: Transfection preformed with pSV3-SV40-T antigen plasmid DNA
BioSafety Level: II
Species: Mouse
Source Organ: Marrow from long bone
Growth Properties: Adherent
Morphology: Polygonal
Population Doubling: N/A
Seeding Density: Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Markers: N/A
Donor Age: N/A
Donor Gender: N/A
Donor Ethnicity: N/A
Propagation: The base medium for this cell line is Prigrow III medium available at abm (TM003). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to final concentration of 6%, 6% Nu-Serum (Corning™ 355100), and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: +37.0°C. * abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.
Quality Control: RT-PCR was used to detect the presence of SV40 large T antigen and CD81
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C