Immortalized Mouse CD81+/− Macrophage Cells (2BSD1.10)

Description: Immortalized Mouse CD81−/− Macrophage Cells (ASD1) (Cat. No. T0138) is established from the long bones of CD81 -/- mice, which were immortalized with the SV40 large T antigen and underwent limiting dilution cloning. Immortalized Mouse CD81+/− Macrophage Cells (2BSD1.10) was generated from transfecting the ASD1 cells with the pCDNA3.1/Hygro vector carrying the CD81 gene. The cell line offers users the ability to conduct experiments with a reliable source of immortal cells in vitro. The CD81 gene has associations with malignancies as it is localized within the tumor-suppressor gene region. Hence valuable for studies in regards to cancer.

Immortalization Method: Transfection preformed with pSV3-SV40-T antigen plasmid DNA

BioSafety Level: II

Species: Mouse

Source Organ: Marrow from long bone

Growth Properties: Adherent

Morphology: Polygonal

Population Doubling: N/A

Seeding Density: Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers: N/A

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow III medium available at abm (TM003). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to final concentration of 6%, 6% Nu-Serum (Corning^™ 355100), and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.

* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: RT-PCR was used to detect the presence of SV40 large T antigen and CD81

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C