Immortalization Method: Serial passaging and infection with lentivirus carrying hTERT gene
BioSafety Level: II
Organism: Mouse (M. musculus)
Species: Mouse
Source Organs: Blood Vessel
Organ Type: Blood Vessel
Growth Properties: Adherent
Morphology: Endothelial
Population Doubling: 24 - 36 hours
Seeding Density: 30,000 cells/cm^²
Propagation: Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow II (TM002) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255), +37.0°C, 5% CO₂
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C