Description: Immortalized Mouse Cardiac Endothelial Cells (MCEC) were isolated from FVB mouse pup hearts. The cells have normal endothelial phenotype with contact inhibition and are easily transfectable. Immortalized Mouse Cardiac Endothelial Cells (MCEC) are suitable for study of endothelial cell characteristics, the vascular system, and drug development.Immortalization Method: Immortalized via infection with lentivirus containing hTERT and SV40-T antigenBioSafety Level: IISpecies: MouseSource Organ: HeartGrowth Properties: AdherentMorphology: Endothelial-likePopulation Doubling: 20 - 30 hoursSeeding Density: 15,000 - 20,000 cells/cm10²Markers: SV40-T, hTERT, PECAM-1, VE-cadherin, von Willebrand FactorDonor Age: N/ADonor Gender: N/ADonor Ethnicity: N/APropagation: The base medium for this cell line is Prigrow III available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 5%, 1X GlutaMAX (Gibco 35050061), 1X HEPES (Gibco 15630080), 40 µg/mL heparin, 25 µg/mL endothelial cell growth supplement (Sigma E2759), and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. This cell line needs to be cultured in gelatin coated flasks.
Carbon dioxide (CO2): 5%, Temperature: +37.0°C.
* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.Quality Control: 1) Cell incubation and prostaglandin assays; 2) Immunofluorescence studies; 3) Western blot; 4) rtPCR; 5) Immunohistochemistry; 6) Permeability studiesShipping Condition: Dry IceStorage Condition: liquid nitrogen or -180°C