Immortalized Mouse Calvarial Mesenchymal Progenitor Cells (iCALs)

The bone morphogenetic proteins (BMPs) produced by calvarial cells are crucial in osteoblastic differentiation and bone regeneration, however the limited lifespan of primary cells diminishes their usefulness in translational research. The Immortalized Mouse Calvarial Mesenchymal Progenitor Cell Line (iCALs) retains the morphology and progenitor cell profile phenotype of primary calvarial cells in addition to preserving BMP-2 –induced osteogenic differentiation. These cells are effective for research examining potential novel small molecule therapeutic agents, cranial reconstruction, alleviation of congenital defects, or the intracellular processes responsible for intramembranous bone formation.

Immortalization Method: Serial passaging and infection with retrovirus carrying loxP-flanked SV40 large T antigen

BioSafety Level: II

Species: Mouse

Source Organ: Calvariae

Growth Properties: Adherent

Morphology: Fibroblast-like

Population Doubling: N/A

Seeding Density: Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers: CD166, CD73, CD44, CD105

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow III medium available at abm (TM003). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: Immunofluorescent staining for mesenchymal stem cell markers

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C