Immortalized Mouse Brain Microvascular Endothelial Cells - SV40T

Brain Microvascular Endothelial Cells plays an important role of forming the blood-brain barrier that is responsible for protection and homeostasis of the parenchyma. It also plays part for the neurovascular unit which regulates interchange of fluids, molecules and cells between peripheral blood and the central nervious system. The Immortalized Mouse Brain Microvascular Endothelial Cells - SV40T cell line could be useful for cell-cell adhesion tests, migration, vascular tube formation or transendothelial resistatnce (TER).

Immortalization Method: Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen.

BioSafety Level: II

Species: Mouse

Source Organ: Brain

Growth Properties: Adherent

Morphology: Polygonal

Population Doubling: N/A

Seeding Density: N/A

Markers: N/A

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow X series medium available at ABM, Cat. No. TM0901.

Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.

Quality Control: N/A

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C