Immortalized Mouse alpha-GalA-/o Schwann Cells (1089C1)

Description: Immortalized Mouse alpha-GalA-/o Schwann Cells (1089C1) is a spontaneously immortalized cell line from Fabry mice. 1089C1 has a disrupted Gla gene allele and exhibits a distinct Schwann cell morphology and biochemical phenotype (α-Galactosidase activity was deficient). This cell line can be used as a murine model for Fabry disease studies.

Immortalization Method: Spontaneous immortalization

BioSafety Level: II

Species: Mouse

Source Organ: Brain

Growth Properties: Adherent

Morphology: Spindle-shaped

Population Doubling: N/A

Seeding Density: N/A

Markers: S100, laminin, p75NTR, GFAP, globotriaosylceramide (Gb3)

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 5% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Change media every 2-3 days.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.
* Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: 1) RT-PCR; 2) Immunocytochemical analysis; 3) Assaying of α-galactosidase activity and protein
determination

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C