Immortalized Mink Endometrial Stromal Cells (GMms1)

Description: Immortalized Mink Endometrial Stromal Cells (GMms1) are derived from uterus of adult female minks. These cells were found to have a positive effect on enhancing the frequency and duration of embryo survival in vitro when coculturing with mink embryos.

Immortalization Method: Immortalized with vector pBAPSV40TtsA58 containing SV40 large T antigen

BioSafety Level: II

Species: Mink

Source Organ: Uterus

Growth Properties: Adherent

Morphology: Fibroblast-like

Population Doubling: N/A

Seeding Density: N/A

Markers: Neomycin (pSV2neo)

Donor Age: Adult

Donor Gender: Female

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is DMEM and F-10 (Gibco, 1:1). and 10 mg/ml insulin (Sigma). To make make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 5%, Penicillin/Streptomycin Solution (G255) to a final concentration of 1%, estradiol 17β (Sigma) to a final concentration of 10^-7, insulin (Sigma) to a final concentration of 10 mg/mL, fungizone to a final concentration of 0.5 µg/mL.
Change media every 2-3 days.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.
* Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: 1) Immunocytochemistry

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C