Immortalized MEF Psen1/2^-/- Cells

MEF Psen1/2^-/- cells were derived from E8.5 embryos which were PSEN negative, and is neomycin and hygromycin-resistant. The cells were immortalized with SV40 large T antigen via transfection with the pMSSVLT plasmid, and were continuously grown in culture to established the immortalized cell line.

Immortalization Method: Immortalized with pMSSVLT plasmid carrying SV40 large T antigen

BioSafety Level: II

Organism: Mouse (M. musculus)

Species: Mouse

Source Organs: Embryo

Organ Type: Embryo

Growth Properties: Adherent

Morphology: Fibroblast-like

Markers: Psen1/2^-/-Neomycin (G418) and hygromycin-resistant

Propagation: The base medium for this cell line is Prigrow IV medium available at abm, Cat. No. TM004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10%, and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.Change media every 2-3 days.Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.* Do not use heat-inactivated FBS for cell culture unless specified otherwise. Recommend split ratio of 1:15 - 1:20 in new cell culture flask twice a week.

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C

Reference: Herreman, A., Van Gassen, G., Bentahir, M., Nyabi, O., Craessaerts, K., Mueller, U., Annaert, W., & De Strooper, B. (2003). gamma-Secretase activity requires the presenilin-dependent trafficking of nicastrin through the Golgi apparatus but not its complex glycosylation. Journal of cell science, 116(Pt 6), 1127–1136. https://doi.org/10.1242/jcs.00292Veugelen, S., Saito, T., Saido, T. C., Chávez-Gutiérrez, L., & De Strooper, B. (2016). Familial Alzheimer's Disease Mutations in Presenilin Generate Amyloidogenic Aβ Peptide Seeds. Neuron, 90(2), 410–416. https://doi.org/10.1016/j.neuron.2016.03.010