Immortalized Mast Cells (NCL-2)

Most immortalized mast cell lines are tumour cell lines, and carry a gain-of-function mutation of Kit. Immortalized Mast Cells (NCL-2) is derived from NC mouse bone marrow, and does not carry a Kit mutation. NLC-2 can respond to exogenous growth signals SCF and IL-3, and releases various chemical mediators against several stimuli including crosslinking of Fc εRI. This cell line could be a useful tool to analyze proliferation, differentiation, and function of normal mast cells.

Immortalization Method: Spontaneous

BioSafety Level: II

Species: Mouse

Source Organ: Bone Marrow

Growth Properties: Suspension

Morphology: Round

Population Doubling: Approximately 30 hours

Seeding Density: 150,000 - 200,000 cells/mL

Markers: Fc εRI, KIT

Donor Age: N/A

Donor Gender: Male

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow VIII medium available at abm, Cat. No. TM018> or Prigrow II medium available at abm, Cat. No. TM002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10%, Penicillin/Streptomycin Solution (G255) to a final concentration of 1%, β-mercaptoethanol to a final concentration of 50 µM, L-glutamine (G275) to a final concentration of 2 mM, and Recombinant Mouse IL3 (Z200145) to a final concentration of 5 ng/mL.
Carbon dioxide (CO₂): 5%, Temperature: 33.0°C.
* Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: 1) Flow cytometry

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C