Description: Most immortalized mast cell lines are tumour cell lines, and carry a gain-of-function mutation of Kit. Immortalized Mast Cells (NCL-2) is derived from NC mouse bone marrow, and does not carry a Kit mutation. NLC-2 can respond to exogenous growth signals SCF and IL-3, and releases various chemical mediators against several stimuli including crosslinking of Fc εRI. This cell line could be a useful tool to analyze proliferation, differentiation, and function of normal mast cells.Immortalization Method: SpontaneousBioSafety Level: IISpecies: MouseSource Organ: Bone MarrowGrowth Properties: SuspensionMorphology: RoundPopulation Doubling: Approximately 30 hoursSeeding Density: 150,000 - 200,000 cells/mLMarkers: Fc εRI, KITDonor Age: N/ADonor Gender: MaleDonor Ethnicity: N/APropagation: The base medium for this cell line is Prigrow VIII medium available at abm, Cat. No. TM018 or Prigrow II medium available at abm, Cat. No. TM002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10%, Penicillin/Streptomycin Solution (G255) to a final concentration of 1%, β-mercaptoethanol to a final concentration of 50 µM, L-glutamine (G275) to a final concentration of 2 mM, and Recombinant Mouse IL3 (Z200145) to a final concentration of 5 ng/mL.
Carbon dioxide (CO2): 5%, Temperature: 33.0°C.
* Do not use heat-inactivated FBS for cell culture unless specified otherwise.Quality Control: 1) Flow cytometryShipping Condition: Dry IceStorage Condition: liquid nitrogen or -180°C