Immortalized Human Vascular Endothelial Cells (EA.hy926) was developed via fusion between a HUVE cell with an A549/8 cell. The cells express Weibel-Pa]ade bodies, and is a valuable experimental model to study this organelle of the endothelium.
Immortalization Method: Spontaneous
BioSafety Level: II
Organism: Human
Species: Human
Source Organs: Human Umbilical Vein Endothelial Cells
Organ Type: Human Umbilical Vein Endothelial Cells
Growth Properties: Adherent
Morphology: Polygonal
Markers: von Willebrand factor (vWF), GMP-140, prostacyclin, platelet activating factor, tissue plasminogen activator, plasminogen activator inhibitor type I, thrombomodulin, vitronectin receptor
Propagation: The base medium for this cell line is Prigrow III medium available at
abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999) to a final concentration of 10%, 100
µM hypoxanthine, 0.4
µM aminopterin, and 16
µ;M thymidine (HAT), and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.Change media every 2-3 days.Carbon dioxide (CO
2): 5%, Temperature: +37.0°C.
Quality Control: 1) ELISA for vWR
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C
Reference: Edgell, C.-J. S., Haizlip, J. E., Bagnell, C. R., Packenham, J. P., Harrison, P., Wilbourn, B., & Madden, V. J. (1990). Endothelium specific Weibel-Palade bodies in a continuous human cell line, EA.hy926.
in vitro Cellular & Developmental Biology, 26(12), 1167–1172. https://doi.org/10.1007/bf02623694