Immortalization Method: Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen
BioSafety Level: II
Species: Human
Source Organ: Umbilical Cord
Growth Properties: Adherent
Morphology: Polygonal
Population Doubling: 75-110 hours
Seeding Density: Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Markers: N/A
Donor Age: N/A
Donor Gender: N/A
Donor Ethnicity: N/A
Propagation: The base medium for this cell line is Prigrow X series medium available at abm, Cat. No. TM0013. Carbon dioxide (CO2): 5%, Temperature: +37.0°C
Quality Control: Real Time PCR was used to quantify the transgene expression.
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C