Immortalized Human Total Liver Cell Population

Immortalization Method: Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen

BioSafety Level: II

Species: Human

Source Organ: Liver

Growth Properties: Adherent with some suspension cells

Morphology: Other

Population Doubling: 48 - 55 hours

Seeding Density: 30,000-40,000 cells/cm²; Recommend split ratio is 1:2 to 1:3

Markers: N/A

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. This cell line needs to be cultured in collagen coated flasks.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C. * abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: Real Time PCR was used to quantify the transgene expression.

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C