Immortalized Human T Cells were generated via lentiviral transduction of T cells isolated from a healthy donor
Immortalization Method: Transduction with Lenti-hTERT (LV616) lentivirus, Lenti-Rb siRNA (LV621) lentivirus, and a mixture of ten lentiviruses (ID1, ID2, ID3, Nanog, EZH2, Fos, SOX2, YAP1, LMO2 and KLF4)
BioSafety Level: II
Organism: Human (H. sapiens)
Species: Human
Source Organs: Blood
Organ Type: Blood
Growth Properties: Suspension
Morphology: Round
Propagation: PriGrow X Series Medium (TM0086) + 1% Penicillin/Streptomycin Solution (G255), +37.0°C, 5% CO₂
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C