Immortalization Method: Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen
BioSafety Level: II
Species: Human
Source Organ: Synovial Membrane
Growth Properties: Adherent
Morphology: Multipolar
Population Doubling: N/A
Seeding Density: 7,000 - 10,000 cells per cm10²
Markers: N/A
Donor Age: 40
Donor Gender: Female
Donor Ethnicity: Caucasian
Propagation: The base medium for this cell line is Prigrow III medium available at
abm, Cat. No.
TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (
TM999)* to a final concentration of 10%, 1% L-glutamine (
G275) to a final concentration of 2mM, and Penicillin/Streptomycin Solution (
G255) to a final concentration of 1%. Carbon dioxide (CO
2): 5%, Temperature: +37.0°C.
* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.Quality Control: Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C