BioSafety Level: IISpecies: HumanSource Organ: CartilageGrowth Properties: AdherentMorphology: PolygonalSeeding Density: Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.Propagation: Grow cells in T25 ECM-coated flasks(G299) with the following conditions. Grow cells in T25 ECM-coated flasks(G299) with the following conditions. The base medium for this cell line is Prigrow X series medium available at abm, Cat. No. TM0032. Carbon dioxide (CO2): 5%, Temperature: 37.0°C.Quality Control: Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.Shipping Condition: Dry IceStorage Condition: liquid nitrogen or -180°C