Immortalization Method: N/A
BioSafety Level: II
Species: Human
Source Organ: Cartilage
Growth Properties: Adherent
Morphology: Polygonal
Population Doubling: N/A
Seeding Density: Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Markers: N/A
Donor Age: N/A
Donor Gender: N/A
Donor Ethnicity: N/A
Propagation: Grow cells in T25 ECM-coated flasks(G299) with the following conditions. Grow cells in T25 ECM-coated flasks(G299) with the following conditions. The base medium for this cell line is Prigrow X series medium available at abm, Cat. No. TM0032. Carbon dioxide (CO2): 5%, Temperature: +37.0°C.
Quality Control: Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C