The small airways epithelial cells form a continuous lining to the airways play a unique role as a protective physical and functional barrier to external deleterious agents. These cells function in the regulation of immune responses by contributing to host defense through chemokine production, adhesion molecule expression, and possibly antigen presentation via HLA-DR expression. These cells provide an ideal model for the studies of toxicity, cystic fibrosis, asthma, pathogenesis, pharmacology or airway wound healing.Immortalization Method: Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen BioSafety Level: IISpecies: HumanSource Organ: LungGrowth Properties: AdherentMorphology: PolygonalPopulation Doubling: N/ASeeding Density: 10,000 - 15,000 cells per cm²Markers: CytokeratinDonor Age: 46Donor Gender: MaleDonor Ethnicity: CaucasianPropagation: The base medium for this cell line is Prigrow I medium available at abm, Cat. No. TM001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10%, 2mM L-glutamine (G275), and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: +37.0°C. * abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.Quality Control: Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.Shipping Condition: Dry IceStorage Condition: liquid nitrogen or -180°C