Immortalization Method: Serial passaging and transduction with recombinant lentiviruses carrying hTERT gene
BioSafety Level: II
Species: Human
Source Organ: Skin
Growth Properties: Adherent
Morphology: Bipolar
Population Doubling: around 30 hours
Seeding Density: Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Markers: N/A
Donor Age: N/A
Donor Gender: N/A
Donor Ethnicity: N/A
Propagation: The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: +37.0°C. * abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.
Quality Control: Real Time PCR was used to quantify hTERT gene expression in immortalized cell line.
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C