Immortalization Method: Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigenBioSafety Level: IISpecies: HumanSource Organ: Skeletal MuscleGrowth Properties: AdherentMorphology: MultipolarPopulation Doubling: 24-34 hoursSeeding Density: 20,000-30,000 cells/cm10²; Recommended split ratio is 1:2 to 1:3Markers: DesminDonor Age: 7Donor Gender: MaleDonor Ethnicity: CaucasianPropagation: The base medium for this cell line is Prigrow III medium available in abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Split Ratio: 1:2 or 1:3 recommended when subculturing. Carbon dioxide (CO2): 5%, Temperature: +37.0°C.
* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.Quality Control: Real Time PCR was used to quantify the transgene expression.Shipping Condition: Dry IceStorage Condition: liquid nitrogen or -180°C