Immortalization Method: Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen
BioSafety Level: II
Species: Human
Source Organ: Skeletal Muscle
Growth Properties: Adherent
Morphology: Multipolar
Population Doubling: 30-40 hours
Seeding Density: 20,000-35,000 cells/cm10²; Recommended split ratio is 1:2 to 1:3
Markers: N/A
Donor Age: 7
Donor Gender: Male
Donor Ethnicity: N/A
Propagation: The base medium for this cell line is Prigrow III medium available in
abm, Cat. No.
TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (
TM999)* to a final concentration of 10% and Penicillin/Streptomycin Solution (
G255) to a final concentration of 1%. Split Ratio: 1:2 or 1:3 recommended when subculturing. Carbon dioxide (CO
2): 5%, Temperature: +37.0°C.
* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.Quality Control: Real Time PCR was used to quantify the transgene expression.
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C