Immortalized Human Scalp Dermal Papilla Cells were isolated from scalp hair follicles from a male with androgenetic alopecia. These cells are applicable for studies of hair growth, hair morphogenesis, and hair cycling.Immortalization Method: Co-transfection with vectors carrying SV40 & hTERT genesBioSafety Level: IISpecies: HumanSource Organ: SkinGrowth Properties: AdherentMorphology: Mulit-polarPopulation Doubling: 24 - 30 hoursSeeding Density: N/AMarkers: Neomycin, Hygromycin, Wnt5a, ALP, HEY1, HEY2, FGF7Donor Age: 35 years oldDonor Gender: Male Donor Ethnicity: KoreanPropagation: The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10%, and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.Carbon dioxide (CO2): 5%, Temperature: +37.0°C.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.Quality Control: 1) RT-PCR; 2) ImmunocytochemistryShipping Condition: Dry IceStorage Condition: liquid nitrogen or -180°C