Description: Immortalized Human Rheumatoid Arthritis Cartilage Progenitor Cells (RA-CPC) are derived from osteoarthritic knee cartilage of a caucasian female. The cells were immortalized by inducing the cells with hTERT. The cells are progenitors cells positive for mesenchymal stem cell (MSC) markers, and may also be differentiated into adipocytes and cells of the osteoblastic lineage for further studies.Immortalization Method: Immortalized with lentivirus containing hTERT, packaged using Lentifectin™ Transfection Reagent (G074)BioSafety Level: IISpecies: HumanSource Organ: CartilageGrowth Properties: AdherentMorphology: Spindle-shapedPopulation Doubling: 28 - 34 hoursSeeding Density: 1,000 cells/cm10²; recommended split ratio of 1:2 to 1:3Markers: Stem cell markers CD29, CD44, CD73, CD90, CD105 (weak expression)Donor Age: N/ADonor Gender: FemaleDonor Ethnicity: CaucasianPropagation: The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1% or 500µl gentamycin (Roth, HN09.1, 50mg/ml).
Change media every 2-3 days.
Carbon dioxide (CO2): 5%, Temperature: +37.0°C.
* Do not use heat-inactivated FBS for cell culture unless specified otherwise.Quality Control: 1) FACSShipping Condition: Dry IceStorage Condition: liquid nitrogen or -180°C