Immortalized Human Renal Proximal Tubule Cells

Immortalization Method: Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen

BioSafety Level: II

Species: Human

Source Organ: Renal

Growth Properties: Adherent

Morphology: Polygonal

Population Doubling: N/A

Seeding Density: 15,000 – 25,000 cells/cm²; Recommended split ratio is 1:2 to 1:3

Markers: Neomycin

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Change media every 2-3 days.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.
* Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: Real Time PCR was used to quantify the transgene expression.

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C