Isolated from adult human lung tissue, their principle function is production of type III collagen, elastin, and proteoglycans of the extracellular matrix of the alveolar septa. Pulmonary fibroblasts play an important role in the repair and remodeling processes following injury. Fibroblast disorders like pulmonary fibrosis or hypoxia-induced pulmonary hypertension can also be investigated with cultured human primary pulmonary fibroblasts.Immortalization Method: Serial passaging and transduction with recombinant lentiviruses carrying a SV40 gene BioSafety Level: IISpecies: HumanSource Organ: LungGrowth Properties: AdherentMorphology: MultipolarPopulation Doubling: 60-70 hoursSeeding Density: 40,000 – 50,000 cells/cm²Markers: N/ADonor Age: 65Donor Gender: FemaleDonor Ethnicity: CaucasianPropagation: The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: +37.0°C. * abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.Quality Control: Real Time PCR was used to quantify transgene expression.Shipping Condition: Dry IceStorage Condition: liquid nitrogen or -180°C