Immortalization Method: Serial passaging and transduction with recombinant lentiviruses carrying SV40 antigenBioSafety Level: IISpecies: HumanSource Organ: HeartGrowth Properties: AdherentMorphology: MultipolarPopulation Doubling: 35-40hoursSeeding Density: 5000 cells/cm²Markers: N/ADonor Age: N/ADonor Gender: N/ADonor Ethnicity: N/APropagation: The base medium for this cell line is Prigrow II medium available at abm, Cat. No. TM002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 5% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: +37.0°C. * abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.Quality Control: Real Time PCR was used to quantify the transgene expression.Shipping Condition: Dry IceStorage Condition: liquid nitrogen or -180°C