Immortalized Human Pterygium Fibroblast - SV40

Immortalization Method: Serial passaging and transduction with replication incompetent recombinant lentiviruses carrying SV40 Large T antigen

BioSafety Level: II

Species: Human

Source Organ: Eye

Growth Properties: Adherent

Morphology: Polygonal

Population Doubling: 36-50 hours

Seeding Density: Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers: N/A

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow X.1 series medium available at abm, Cat. No. TM010.

Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.

Quality Control: Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C