Immortalized Human Primary Trabecular Meshwork Cells - SV40

Immortalized Human Primary Trabecular Meshwork Cells - SV40 were derived from the normal juxtacanalicular and corneoscleral tissue regions of the human eye, and were immortalized with SV40. Recommended for use in research for glaucoma and studies involving trabecular meshwork functions.

Immortalization Method: Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen

BioSafety Level: II

Species: Human

Source Organ: Juxtacanalicular and corneoscleral regions of human eye

Growth Properties: Adherent

Morphology: Polygonal

Population Doubling: 18-25 hours

Seeding Density: 10,000 - 20,000 cells/cm²

Markers: N/A

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C