Immortalized Human Periodontal Ligament Fibroblasts - HPV

Immortalization Method: Transduction with lentiviral HPV-16 E6/E7 (abm Cat. No. LV617)

BioSafety Level: II

Species: Human

Source Organ: Periodontal Ligament

Growth Properties: Adherent

Morphology: Multipolar

Population Doubling: 30-40 hours

Seeding Density: 5,000 - 10,000 cells/cm10²

Markers: N/A

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is PriGrow II medium available at abm, Cat. No. TM002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999) to a final concentration of 10%, and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.

Quality Control: Real Time PCR was used to quantify the transgene expression.

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C