Immortalized Human Periodontal Ligament Fibroblasts - HPV

Immortalization Method: Transduction with lentiviral HPV-16 E6/E7 (abm Cat. No. LV617)

BioSafety Level: II

Species: Human

Source Organ: Periodontal Ligament

Growth Properties: Adherent

Morphology: Multipolar

Population Doubling: 30-40 hours

Seeding Density: 5,000 - 10,000 cells/cm²

Markers: N/A

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is PriGrow II medium available at abm, Cat. No. https://new.abmgood.com/Prigrow-II-Medium-TM002.html">TM002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (https://new.abmgood.com/USDA-Research-Grade-Origin-Fetal-Bovine-Serum-TM999-500.html">TM999) to a final concentration of 10%, and Penicillin/Streptomycin Solution (https://new.abmgood.com/Penicillin/Streptomycin-Solution-G255.html#g255">G255) to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.

Quality Control: Real Time PCR was used to quantify the transgene expression.

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C