Description: The Immortalized Human Primary Pancreatic cells are derived from Pancreatic β cells, an important component of pancreas which make up 65-80% of the cells in the islets of Langerhans. They are essential in blood glucose regulation by producing and secreting insulin, the critical hormone that regulates the body to reduce blood glucose.These cells are ideal models to provide insight into development of novel therapies for Type 1 diabetes and other pancreas related diseases.Immortalization Method: Serial passaging and transduction with recombinant lentiviruses carrying c-Myc geneBioSafety Level: IISpecies: HumanSource Organ: PancreasGrowth Properties: AdherentMorphology: PolygonalPopulation Doubling: N/ASeeding Density: Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.Markers: N/ADonor Age: N/ADonor Gender: N/ADonor Ethnicity: N/APropagation: The base medium for this cell line is Prigrow II medium available at abm, Cat. No. TM002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. This cell line needs to be cultured in collagen coated flasks. Carbon dioxide (CO2): 5%, Temperature: +37.0°C.
* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.Quality Control: Real Time PCR was used to quantify Myc gene expression in immortalized cell line.Shipping Condition: Dry IceStorage Condition: liquid nitrogen or -180°C