Description: Immortalized Human Osteoarthritis Cartilage Mesenchymal Stem Cells (OASC5) are derived from knee articular cartilage of male and female osteoarthritis (OA) patients. The cells contribute to pathogenesis of cartilage degeneration and can be served as a novel cell target for OA therapy.Immortalization Method: Immortalized with the vector pRetro-E2 containing SV40 large T antigenBioSafety Level: IISpecies: HumanSource Organ: CartilageGrowth Properties: AdherentMorphology: Fibroblast-likePopulation Doubling: N/ASeeding Density: N/AMarkers: ALCAM (CD166)Donor Age: AdultDonor Gender: Pooled from male and female OA patientsDonor Ethnicity: N/APropagation: The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10%, Penicillin/Streptomycin Solution (G255) to a final concentration of 1%, HEPES to a final concentration of 100 mM, L-glutamine (G275) to a final concentration of 2 mM, ascorbic acid to a final concentration of 0.1 mM, Sodium Pyruvate Solution (TM057) to a final concentration of 0.1 mM, and L-glucose to a final concentration of 2.7 μM.
Change media every 2-3 days.
Carbon dioxide (CO2): 5%, Temperature: +37.0°C.
* Do not use heat-inactivated FBS for cell culture unless specified otherwise.Quality Control: 1) qPCR; 2) Flow cytometry; 3) Differentiation assays; 4) ImmunocytochemistryShipping Condition: Dry IceStorage Condition: liquid nitrogen or -180°C