Immortalization Method: N/A
BioSafety Level: II
Species: Human
Source Organ: Human Oral Mucosa
Growth Properties: Adherent
Morphology: Other
Population Doubling: N/A
Seeding Density: Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Markers: N/A
Donor Age: N/A
Donor Gender: N/A
Donor Ethnicity: N/A
Propagation: Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions.The base medium for this cell line is the Keratinocyte Medium Kit available at abm, Cat. No TM040. Carbon dioxide (CO2): 5%, Temperature: +37.0°C.
Quality Control: Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C