Description: Immortalized Human Oral Gingival Keratinocytes (HPV-16 GM) are derived from a healthy donor and were immortalized using HPV16 E6/E7 genes. Immortalized Human Oral Gingival Keratinocytes (HPV-16 GM) express the following typical epithelial marker genes: cytokeratin 1, cytokeratin 10, involucrin, and filaggrin.Immortalization Method: Immortalized with retrovirus containing the E6/E7 genes of HPV16BioSafety Level: IISpecies: HumanSource Organ: GingivaGrowth Properties: AdherentMorphology: CobblestonePopulation Doubling: 44 - 54 hoursSeeding Density: 5700 cells/cm10²Markers: IL-1β, GMCSF, FGF-7, EGFDonor Age: N/ADonor Gender: N/ADonor Ethnicity: N/APropagation: The base medium for this cell line is Prigrow X medium available at abm, Cat. No. TM0717.
Carbon dioxide (CO2): 5%, Temperature: +37.0°C.
* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.Quality Control: 1) Indirect immunofluorescence; 2) RT PCR; 3) Transplantation assayShipping Condition: Dry IceStorage Condition: liquid nitrogen or -180°C