Immortalized Human Oral Gingival Keratinocytes (HPV-16 GM) are derived from a healthy donor and were immortalized using HPV16 E6/E7 genes. Immortalized Human Oral Gingival Keratinocytes (HPV-16 GM) express the following typical epithelial marker genes: cytokeratin 1, cytokeratin 10, involucrin, and filaggrin.
Immortalization Method: Immortalized with retrovirus containing the E6/E7 genes of HPV16
BioSafety Level: II
Species: Human
Source Organ: Gingiva
Growth Properties: Adherent
Morphology: Cobblestone
Population Doubling: 44-54 hours
Seeding Density: 5700 cells/cm²
Markers: IL-1β, GMCSF, FGF-7, EGF
Donor Age: N/A
Donor Gender: N/A
Donor Ethnicity: N/A
Propagation: The base medium for this cell line is Prigrow X medium available at abm, Cat. No. TM0717. Carbon dioxide (CO2): 5%, Temperature: +37.0°C.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.
Quality Control: 1) Indirect immunofluorescence; 2) RT PCR; 3) Transplantation assay
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C