Microglia cells are resident macrophages of the brain and spinal cord and they act as the first and the main form of active immune defense in the nervous system. The Immortalized Human Microglia - SV40 cell line is derived from Primary Human Microglia Cells (>99% purity) and have maintained microglial marker CD68 and NGF as demonstrated by RT-PCR. These cells are suitable for studies of human microglia in health and disease.
Immortalization Method: Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen
BioSafety Level: II
Species: Human
Source Organ: Brain
Growth Properties: Adherent
Morphology: Polygonal
Population Doubling: 50-60 hours
Seeding Density: 35,000 – 40,000 cells/cm²; Recommended split ratio is 1:2 to 1:3
Markers: N/A
Donor Age: N/A
Donor Gender: N/A
Donor Ethnicity: N/A
Propagation: The base medium for this cell line is Prigrow III medium available at
abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10%, and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.Carbon dioxide (CO
2): 5%, Temperature: +37.0°C.
* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.Quality Control:- Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C