Immortalized Human Mesothelial Cells (HPV E6E7)

The Immortalized Human Mesothelial cells - HPV E6/E7 are derived from the normal human mesothelium, an important structure lining human abdomen cavities to provide lubrication, transportation and movement of fluids, immune cells, cytokines and proteins. These specialized cells are critical in regulating inflammatory responses and innate immune systems to protect against infections and tumor propagation. Their roles in cancer metastasis, metabolic diseases, peritoneal dialysis and surgical adhesions make them an ideal model for cancer research, pathological and/or therapeutic study.

Immortalization Method: Serial passaging and transduction with recombinant lentiviruses carrying HPV E6/E7 gene

BioSafety Level: II

Species: Human

Source Organ: Mesentery

Growth Properties: Adherent

Morphology: Polygonal

Population Doubling: 40 - 50 hours

Seeding Density: 15,000 - 20,000 cells/cm²

Markers: APOBEC3, CD105, VEGF

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow X series medium available at abm, Cat. No. TM0456.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C

Quality Control: Real Time PCR was used to quantify HPV E6 and E7 gene expression in immortalized cell line.

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C