Immortalization Method: Serial passaging and transduction with recombinant lentiviruses carrying HPV E6/E7 geneBioSafety Level: IISpecies: HumanSource Organ: RenalGrowth Properties: AdherentMorphology: PolygonalPopulation Doubling: 144-168 hoursSeeding Density: 50,000-60,000 cells/cm²Markers: cytokeratin 19, fibronectinDonor Age: N/ADonor Gender: N/ADonor Ethnicity: N/APropagation: The base medium for this cell line is Prigrow X series medium available at abm, Cat. No. TM0612. Carbon dioxide (CO2): 5%, Temperature: +37.0°C.Quality Control: Real Time PCR was used to quantify the transgene expression.Shipping Condition: Dry IceStorage Condition: liquid nitrogen or -180°C