Immortalized Human Mammary MUC+ESA+ Epithelial Cells

Immortalized Human Mammary MUC+ESA+ Epithelial Cells are luminal-derived epithelial cells coexpressing sialomucin (MUC) and epithelial-specific antigen (ESA). It has tight junction proteins, occludin, claudin-1, and the ability to build high transepithelial electrical resistance which is all characteristics of differentiated luminal epithelial phenotypes. It can form acinus-like spheres when cultures on laminin rich gel. These cells have characteristics of differentiated mature human breasts.

Immortalization Method: Serial passaging and transduction with human papilloma virus (HPV)-16 E6/E7

BioSafety Level: II

Species: Human

Source Organ: Mammary Gland

Growth Properties: Adherent

Morphology: Polygonal

Population Doubling: N/A

Seeding Density: Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers: CK18, E-cadherin, G418 resistance

Donor Age: 22

Donor Gender: Female

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow III:Prigrow IV (3:1 ratio) medium available at abm (TM003 and TM004). To make the completed growth medium, add the following components to the base medium to final concentration of: 5% fetal bovine serum (TM999)*, 0.5 µg/ml hydrocortisone, 50 µg/ml gentamicin (G272), 5 µg/ml insulin (Z101065), 10 ng/ml EGF (Z100135), 10-10 M cholera toxin, 1.8 x 10-4 M adenine, 10 µM Y27632, 2 mM L-glutamine (G275), and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: Immunocytochemical staining used to determine cell traits

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C