Immortalized Human Mammary Fibroblasts (HMF3S)

Immortalized Human Mammary Fibroblasts (HMF3S) were generated through the combined transduction of mammary microvascular endothelia cells with a temperature sensitive mutant (U19tsA58) of the SV40 Large T Antigen and a catalytic subunit of human telomerase (hTERT). The HMF3S cells grow best at a temperature of 37⁰C and are not temperature sensitive compared to the Immortalized Human Mammary Fibroblasts (HMF3A) (abm, T0153).

Immortalization Method: Combined transduction with retroviruses carrying a temperature sensitive mutant (U19tsA58) of the SV40 Large T Antigen and a catalytic subunit of human telomerase (hTERT)

BioSafety Level: II

Species: Human

Source Organ: Mammary Tissue

Growth Properties: Adherent

Morphology: Spindle shaped

Population Doubling: N/A

Seeding Density: Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers: N/A

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow III medium available at abm (TM003). To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10%, 1% L-glutamine (G275), and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37°C.
The cells must be split 1:5 twice a week.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: 1.) Telomerase-detection assay was used to detect telomerase activity.
2.) Flow cytometry confirmed expression of T antigen.

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C