Immortalized Human Mammary Epithelial Cells (HMEC 2.6)

Immortalized Human Mammary Epithelial Cells (HMEC 2.6) is an immortalized breast epithelial cell line which are not tumorigenic and do not show no anchorage-independent growth. There is a normal distribution of cells in G1, S, and G2/M phase and they express normal breast epithelial markers ( E-cadherin, CK7/18, and CK5/14). The cells retain primary characteristics and are valuable for research relating to mammary epithelial biology.

Immortalization Method: Serial passaging and transduction with LXSN-based retroviruses carrying hTERT and a mutant CDK4(R24C)

BioSafety Level: II

Species: Human

Source Organ: Mammary Tissue

Growth Properties: Adherent

Morphology: Polygonal

Population Doubling: Mean doubling time of around 72 hours.

Seeding Density: 10,000 - 15,000 cells/cm²

Markers: E-cadherin, EGFR, MIB1, CD10, CK5/14, P63, CK7/18, G418 resistance

Donor Age: N/A

Donor Gender: Female

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow IV (TM004). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 1% or 35 µg/ml bovine pituitary extract (Hammond), 12.5 ng EGF (Z100135), 50 µM fresh ascorbic acid, 2 nM estradiol, 1 µg/ml insulin, 2.8 µM hydrocortisone, 0.1 mM ethanolamine, 0.1 mM L-glutamine (G275), 15 nM sodium selenite, and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. For selection use G418 at 100 µg/ml and Zeocin at 20 µg/ml. Adjust media pH to 7.4.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: 1) Cell markers were probed via immunohistochemical analysis; 2) Soft agar assay preformed to determine anchorage-independence.

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C