Immortalization Method: Serial passaging and transduction with recombinant lentiviruses carrying SV40 gene.BioSafety Level: IISpecies: HumanSource Organ: Mammary GlandGrowth Properties: AdherentMorphology: PolygonalPopulation Doubling: N/ASeeding Density: 10,000 - 15,000 cells per cm²Markers: CD10+, MUC1-, cytokeratin; note that these cells lack drug-resistance markersDonor Age: 23Donor Gender: FemaleDonor Ethnicity: CaucasianPropagation: The base medium for this cell line is Prigrow IV medium available at abm, Cat. No. TM004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: +37.0°C. * abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.Quality Control: Real Time PCR was used to quantify the transgene expression.Shipping Condition: Dry IceStorage Condition: liquid nitrogen or -180°C