Immortalized Human Lymphoblastoid Cells – EBV (KMS-9)

The Immortalized Human Lymphoblastoid Cells- EBV (KMS-9) was derived from peripheral human blood lymphocytes, with minimal genetic and phenotypic alterations compared to the parental lymphocytes. Being an actively proliferating B cell population, these cells provide a constant supply of starting materials suitable for various assays and in vitro model system for the study of human B cells.


The KMS-9 cells express HLA-DR on its surface. It also exhibit slight expression of the CD19 B cell marker, but on the other hand, it is negative for CD20. Another lyphoblastoid cell line, KMS-15 (Cat#T0133), in contrast, expresses both CD19B and CD20 cell marker.

Immortalization Method: Epstein-Barr Virus (EBV)

BioSafety Level: II

Species: Human

Source Organ: Peripheral blood

Growth Properties: Suspension

Morphology: Round|Clusters

Population Doubling: N/A

Seeding Density: Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers: N/A

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow II medium available at abm (TM002). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: N/A

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C