Cells were derived from the right posterior superior surface of the true vocal cord
Immortalization Method: Transduction with lentivirus encoding human papillomavirus (HPV) E6/E7
BioSafety Level: II
Organism: Human (H. sapiens)
Species: Human
Source Organs: Airway/Lung
Organ Type: Airway/Lung
Growth Properties: Adherent
Morphology: squamous epithelioid
Population Doubling: 72 hours
Seeding Density: Recommended split ratio is 1:4
Markers: Cytokeratins 5 and 14 (detect by IF)
Donor Age: 32
Donor Gender: Female
Propagation: Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow X Series Medium (TM0850), +37.0°C, 5% CO₂
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C
Reference: Samuels, T. L., Zimmermann, M. T., Zeighami, A., Demos, W., Southwood, J. E., Blumin, J. H., Bock, J. M., & Johnston, N. (2021). RNA Sequencing Reveals Cancer-Associated Changes in Laryngeal Cells Exposed to Non-Acid Pepsin. The Laryngoscope, 131(1), 121–129. https://doi.org/10.1002/lary.28636